General Cell Based Assays
Abcam Lipid Assay Kit (unsaturated fatty acids)
Lipid Assay Kit (unsaturated fatty acids)
The product is subject to the following: Abcam Restricted Use Statement
ION BIOSCIENCES Thallium-free GIRK Potassium Channel Assay pIONeer kit - 2 plates
ION Biosciences' Thallium-free GIRK Potassium Channel Assay pIONeer kit is the first fluorescence-based potassium channel solution that directly measures potassium flux, enabling you to move away from thallium-containing products. This product features the potassium-sensitive dye, IPG-1 AM, our lowest affinity potassium indicator to measure changes in intracellular potassium concentration. This kit is optimized for GIRK (Kir3.x) screening, and can be used to identify activators and inhibitors of GIRK channels. 2 plate kit.
AMID BIOSCIENCES Amid Biosciences, TEV Protease, Recombinant, His-tag, Site-specific Cleavage, 5000 Units
Recombinant TEV protease with a His-tag is supplied in a 5000 Units format for site-specific cleavage of fusion proteins containing a TEV recognition sequence. The enzyme is widely used to remove affinity tags or solubility tags after recombinant protein expression and purification. The His-tag enables removal of TEV protease after digestion by immobilized metal affinity chromatography. Technical specifications are included below.- Quantity - 5000 Units- Gene - TEV protease- Source - E. coli expression system- Tag - N-terminal His-tag- Includes 1 vial of 20X TEV buffer, composed of 1 M Tris-HCl pH 8.0 and 10 mM EDTA, and 1 vial of 100 mM DTT.- Activity - 10,000 Units/mL. One unit of TEV Protease cleaves more than 85% of 3 ug control substrate in 1 hour at 30 C.- Storage - -20 CThis product is for research use only.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4044531 MELANIN ASSAY KIT - 100T
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES PHOSPHATE ASSAY KIT 500TEST
NC3780597 PHOSPHATE ASSAY KIT 500TEST
ELABSCIENCE Alanine Aminotransferase (ALT/GPT) Activity Assay Kit, 100 Assays
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
American Research Products Inc MYB (Phospho-Ser12) Transcription Factor Activity Assay
MYB (Phospho-Ser12) Transcription Factor Activity Assay
Cell Biolabs Inc CytoSelect™ 24-Well Cell Contraction Assay Kit (Floating Matrix Model), 24 assays
Several culture models exist to study fibroblasts' ability of to reorganize and contract collagen matrices in vitro. In the floating matrix contraction model, a freshly polymerized collagen matrix containing cells is released from the culture dish and allowed to float in culture medium, and contraction occurs in the absence of external mechanical load and without appearance of stress fibers in the cells. The CytoSelect™ 24-Well Cell Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators using the floating matrix model. The kit's proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction. Each kit provides sufficient quantities to perform up to 24 assays in a 24-well plate.
Molecular Devices Assay Kit, Calcium 6, Evaluation, Fluorescence, 96/384/1536-well
The FLIPR Calcium 6 Evaluation Kit provides a homogeneous, fluorescence-based method to detect intracellular calcium changes in cell-based assays. It is formulated for use with compatible fluorescence plate readers and supports multiple microplate formats.
- Homogeneous fluorescence assay for intracellular calcium detection.
- Evaluation kit sized for three assay plates and three QF assay plates.
- Includes 3 vials of component A, 3 vials of component C for QF, and 1 bottle of assay buffer.
- Compatible with 96-well, 384-well, and 1536-well microplates.
- Designed for screening GPCRs, ion channels, and calcium-mediated signaling.
- Each reagent vial is sufficient for one plate, depending on dispenser dead volume.
Molecular Devices FLIPR Calcium Assay Kit, 2-Plate Sample, Fluorescence, Homogeneous
Sample kit for performing fluorescence-based intracellular calcium flux assays. The reagents are formulated for a homogeneous mix-and-read workflow to accelerate assay development and screening of GPCR and ion channel targets on compatible instruments.
- Sample kit containing two Component A vials, each sufficient for one microplate.
- Fluorescence-based, homogeneous assay format for calcium flux detection.
- Optimized for GPCR and ion channel screening and pharmacology studies.
- Compatible with common FLIPR and plate reader systems, including Tetra, Penta, and FlexStation platforms.
- Component B dilution/assay buffer is sold separately and required for some kit sizes.
Molecular Devices Neurotransmitter Uptake Assay Kit, Fluorescence, Live-Cell, 96/384, 50-Plate
The Molecular Devices Neurotransmitter Transporter Uptake Assay Kit is a homogeneous, fluorescence-based live-cell assay for measuring uptake activity of serotonin (SERT), norepinephrine (NET), and dopamine (DAT) transporters. The kit uses a fluorescent substrate and masking dye to enable no-wash, mix-and-read endpoint or kinetic measurements on bottom-read microplate readers.
- Homogeneous, no-wash live-cell assay format.
- Fluorescence-based detection suitable for kinetic or endpoint reads.
- Compatible with 96-well and 384-well black clear-bottom plates.
- Bulk kit contains reagents for 50 plates (5 vials of lyophilized dye/masking mix).
- Reconstituted dye solution stable for limited time; store frozen reagents at -20°C.
Molecular Devices Reporter Assay Kit, Dual Luciferase, 1,000 Reactions
SpectraMax DuoLuc Reporter Assay Kit enables sensitive, quantitative measurement of firefly and Renilla luciferase activities in mammalian cells using flash-type luminescence. The bulk format supports high-throughput workflows and is optimized for use with microplate readers equipped with injectors. Preconfigured reader protocols simplify data acquisition and normalization to internal controls.
- Dual luciferase assay for firefly and Renilla measurements.
- Bulk kit provides reagents for 1,000 reactions.
- Flash-type luminescence chemistry for high sensitivity.
- Optimized for microplate readers with injector capability.
- Enables normalization to an internal control reporter.
- Includes assay reagents; additional passive lysis buffer available separately.